Resources

Reagents

You can obtain plasmid and vector reagents via the nonprofit plasmid repository Addgene.

Jin lab reagents at Addgene

If you cannot obtain reagents through Addgene or have additional questions, please feel free to reach out to us directly at jinoffice@scripps.edu.

Protocols

CRISPR-Cas9 editing efficiency analysis through next-generation sequencing

Whole-cell dissociation from perinatal mouse brains

Nuclei extraction from perinatal mouse brains

High-titer lentiviral vector production and purification

Software and Data

All the computational tools and pipelines are available on Github. We also share our data on the single-cell portal for easy access.

Jin lab Github | single cell portal

In vivo Perturb-seq (Science 2020)

GitHub | GSE157977 | single-cell portal

Massively parallel in vivo Perturb-seq (bioRxiv 2023)

GitHub | GSE249416 | single-cell portal: AAV screen (RNA, AAV barcode) and perturbation screen (RNA, gRNA)